"Induction of pluripotent stem cells from adult human fibroblasts
by defined factors," by Kazutoshi Takahashi, Koji Tanabe, Mari
Ohnuki, Megumi Narita, Tomoko Ischisaka, Kiichiro Tomoda, and Shinya
Yamanaka, Cell, 131(5): 861-72, 30 November 2007.
[Authors' affiliations: Kyoto University, Japan; CREST, Kawaguchi, Japan;
Gladstone Institute of Cardiovascular Disease, San Francisco, CA]
Abstract: "Successful reprogramming of differentiated
human somatic cells into a pluripotent state would allow creation of
patient- and disease-specific stem cells. We previously reported generation
of induced pluripotent stem (iPS) cells, capable of germline transmission,
from mouse somatic cells by transduction of four defined transcription
factors. Here, we demonstrate the generation of iPS cells from adult human
dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and
c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in
morphology, proliferation, surface antigens, gene expression, epigenetic
status of pluripotent cell-specific genes, and telomerase activity.
Furthermore, these cells could differentiate into cell types of the three
germ layers in vitro and in teratomas. These findings demonstrate that iPS
cells can be generated from adult human fibroblasts."
This 2007 report from Cell was cited 88
times in current journal articles indexed by Clarivate
during March-April 2009. No other biology paper published in the last two
years, aside from reviews, collected a higher citation total during that
two-month period. After slipping briefly to #2 last time based on citations
processed during January-February 2009, the paper now regains the #1
standing. Prior to the most recent two-month count, citations have accrued
as follows:
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