"The impact of microRNA on protein output," by Daehyun
Baek, Judit Villen, Chanseok Shin, Fernando D. Camargo, Steven P. Gygi, and
David P. Bartel, Nature, 455(7209): 64-71, 4 September 2008
[Authors' affilations: White Institute for Biomedical Research, Cambridge,
MA; Howard Hughes Medical Institute, MIT, Cambridge, MA; Harvard Medical
School, Boston, MA]
Abstract: "MicroRNAs are endogenous ~ 23-nucleotide RNAs
that can pair to sites in the messenger RNAs of protein-coding genes to
downregulate the expression from these messages. MicroRNAs are known to
influence the evolution and stability of many mRNAs, but their global
impact on protein output had not been examined. Here we use quantitative
mass spectrometry to measure the response of thousands of proteins after
introducing microRNAs into cultured cells and after deleting mir-223 in
mouse neutrophils. The identities of the responsive proteins indicate that
targeting is primarily through seed-matched sites located within favourable
predicted contexts in 39 untranslated regions. Hundreds of genes were
directly repressed, albeit each to a modest degree, by individual
microRNAs. Although some targets were repressed without detectable changes
in mRNA levels, those translationally repressed by more than a third also
displayed detectable mRNA destabilization, and, for the more highly
repressed targets, mRNA destabilization usually comprised the major
component of repression. The impact of microRNAs on the proteome indicated
that for most interactions microRNAs act as rheostats to make fine-scale
adjustments to protein output."
This 2008 report from Nature was cited 43
times in current journal articles indexed by Clarivate
during January-February 2010. No other biology paper published in the last
two years, excluding reviews, collected a higher number of citations during
that two-month period. Prior to the most recent bimonthly count, citations
to the paper have accrued as follows:
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