"Induction of pluripotent stem cells from adult human fibroblasts
by defined factors," by Kazutoshi Takahashi, Koji Tanabe, Mari
Ohnuki, Megumi Narita, Tomoko Ischisaka, Kiichiro Tomoda, and Shinya
Yamanaka, Cell, 131(5): 861-72, 30 November 2007.
[Authors' affiliations: Kyoto University, Japan; CREST, Kawaguchi, Japan;
Gladstone Institute of Cardiovascular Disease, San Francisco, CA]
Abstract: "Successful reprogramming of differentiated
human somatic cells into a pluripotent state would allow creation of
patient- and disease-specific stem cells. We previously reported generation
of induced pluripotent stem (iPS) cells, capable of germline transmission,
from mouse somatic cells by transduction of four defined transcription
factors. Here, we demonstrate the generation of iPS cells from adult human
dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and
c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in
morphology, proliferation, surface antigens, gene expression, epigenetic
status of pluripotent cell-specific genes, and telomerase activity.
Furthermore, these cells could differentiate into cell types of the three
germ layers in vitro and in teratomas. These findings demonstrate that iPS
cells can be generated from adult human fibroblasts."
This 2007 report from Cell was cited 85
times in current journal articles indexed by Clarivate
during September-October 2009. No other biology paper published in the last
two years, aside from reviews, attracted a higher number of citations
during that two-month period. Thus, the paper extends its run of appearing
at either the #1 or #2 spot among biology's most-cited papers for ten
consecutive bimonthly periods (including #1 appearances in the last four
counts). Indeed, it's certain that this paper will exceed the two-year
eligibility limit for Hot Papers and will become too "old" for further
appearances well before its citations begin to wane. Prior to the most
recent two-month tally, citations have accrued as follows:
SOURCE:
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