Sci-Bytes> Hot Paper in Biology
Week of Janaury 2, 2011
"Accurate whole human genome sequencing using reversible terminator chemistry," by David R. Bentley, et al., Nature, 456(7218): 861-72, 30 November 2007.
[Author's affiliations: 7 institutions worldwide]
Abstract: DNA sequence information underpins genetic research, enabling discoveries of important biological or medical benefit. Sequencing projects have traditionally used long (400 - 800 base pair) reads, but the existence of reference sequences for the human and many other genomes makes it possible to develop new, fast approaches to re-sequencing, whereby shorter reads are compared to a reference to identify intraspecies genetic variation. Here we report an approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost. Single molecules of DNA are attached to a flat surface, amplified in situ and used as templates for synthetic sequencing with fluorescent reversible terminator deoxyribonucleotides. Images of the surface are analysed to generate high-quality sequence. We demonstrate application of this approach to human genome sequencing on flow-sorted X chromosomes and then scale the approach to determine the genome sequence of a male Yoruba from Ibadan, Nigeria. We build an accurate consensus sequence from 303 average depth of paired 35- base reads. We characterize four million single-nucleotide polymorphisms and four hundred thousand structural variants, many of which were previously unknown. Our approach is effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
This 2008 report from Nature was cited 37 times in current journal articles indexed by Clarivate during July-August 2010. Thanks to this latest two-month tally, the paper ranks as the third-most-cited biology paper published in the last two years, not counting reviews. Prior to the most recent bimonthly count, citations to the paper have accrued as follows:
May-June 2010: 30 citations
March-April 2010: 31
January-February 2010: 34
November-December 2009: 34
September-October 2009: 40
July-August 2009: 19
May-June 2009: 14
March-April 2009: 10
January-February 2009: 4
November-December 2008: 2
Total citations to date: 255
SOURCE: Hot Papers Database (Included with a subscription to the print newsletter Science Watch®, available from the Research Services Group of Thomson Reuters. Packaged on a CD that is mailed with each Science Watch issue, the Hot Papers Database contains data on hundreds of highly cited papers published during the last two years. User interface permits searching by author, organization, journal, field, and more. Total citations, as well as citations accrued during successive bimonthly periods, can be assessed and graphed. An updated CD containing the most recent bimonthly data is mailed with every new issue of Science Watch, six times a year. The CD also includes an electronic version of the Science Watch issue in HTML format, for personal desktop access.
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