"In vitro reprogramming of fibroblasts into a pluripotent
ES-cell-like state," by Marius Wernig and 7 others, Nature, 448(7151): 318-24, 19 July 2007.
Paste paper here. the source below is for all whats hot in papers
[Authors' affiliations: Whitehead Institute, MIT, Cambridge, MA;
Massachusetts General Hospital, Boston; Broad Institute, Boston; Harvard
Medical School, Boston]
Abstract: "Nuclear transplantation can reprogramme a
somatic genome back into an embryonic epigenetic state, and the
reprogrammed nucleus can create a cloned animal or produce pluripotent
embryonic stem cells. One potential use of the nuclear cloning approach is
the derivation of 'customized' embryonic stem (ES) cells for
patient-specific cell treatment, but technical and ethical considerations
impede the therapeutic application of this technology. Reprogramming of
fibroblasts to a pluripotent state can be induced in vitro through ectopic
expression of the four transcription factors Oct4 (also called Oct3/4 or
Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene
expression and chromatin state of such induced reprogrammed stem cells are
similar to those of ES cells. Notably, the cells--derived from mouse
fibroblasts--can form viable chimaeras, can contribute to the germ line and
can generate live late-term embryos when injected into tetraploid
blastocysts. Our results show that the biological potency and epigenetic
state of in-vitro-reprogrammed induced pluripotent stem cells are
indistinguishable from those of ES cells."
This 2007 report from Nature was cited 40
times in current journal articles indexed by Clarivate
during March-April 2008. With this latest two-month total, the report
currently stands as the third-most-cited biology paper (aside from reviews)
published in the last two years. Prior to the most recent bimonthly count,
citations to the paper have accrued as follows:
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