"Induction of pluripotent stem cells from adult human fibroblasts
by defined factors," by Kazutoshi Takahashi, Koji Tanabe, Mari
Ohnuki, Megumi Narita, Tomoko Ischisaka, Kiichiro Tomoda, and Shinya
Yamanaka, Cell, 131(5): 861-72, 30 November 2007.
[Authors' affiliations: Kyoto University, Japan; CREST, Kawaguchi, Japan;
Gladstone Institute of Cardiovascular Disease, San Francisco, CA]
Abstract: "Successful reprogramming of differentiated
human somatic cells into a pluripotent state would allow creation of
patient- and disease-specific stem cells. We previously reported generation
of induced pluripotent stem (iPS) cells, capable of germline transmission,
from mouse somatic cells by transduction of
four defined transcription factors. Here, we demonstrate the generation of
iPS cells from adult human dermal fibroblasts with the same four factors:
Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human
embryonic stem (ES) cells in morphology, proliferation, surface antigens,
gene expression, epigenetic status of pluripotent cell-specific genes, and
telomerase activity. Furthermore, these cells could differentiate into cell
types of the three germ layers in vitro and in teratomas. These findings
demonstrate that iPS cells can be generated from adult human fibroblasts."
This 2007 report from Cell was cited 59
times in current journal articles indexed by Clarivate
during May-June 2008. Thanks to that latest two-month tally, this is
currently the most-cited biology paper published in the last two years,
aside from reviews. Prior to the most recent bimonthly count, citations to
the paper have accrued as follows:
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